Journal: Ticks and tick-borne diseases

Article Title: A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation

doi: 10.1016/j.ttbdis.2019.101354

Figure Lengend Snippet: (A) Shown is the alignment of partial amino acid sequences of C5 from Peromyscus leucopus mouse (white-footed mouse), Mus musculus mouse (house mouse), rat, human, rabbit, horse, pig, chicken, and quail analyzed by ClustalW. The amino acids of human C5 that make contact with OmCI in the crystal structures shown previously (Jore et al., 2016) are highlighted by red squares. (B) The crystal structure of human C5d (5I5K, residues 932-1372 of human C5) is shown in gray ribbons, and the amino acids involved in binding to OmCI are shown and labeled. The modelled structure of Coturnix quail C5d is indicated (residues 930-1370 of quail C5) as purple ribbons. The quail C5d amino acids corresponding with the residues of human C5d that bind to OmCI are shown and labelled. Graphics were generated using Swiss PDB Viewer (Guex and Peitsch, 1997). The amino acids labelled in yellow are conserved between human and quail C5, whereas the amino acids that vary between these animals’ C5 are shown in red.

Article Snippet: The cell suspensions were then mixed with 40 % of the normal serum from human, Coturnix (quail), Gallus gallus (chickens) (Biowest, Riverside, MO), Anser anser (geese) (BioIVT, Hicksville, NY), Meleagris gallopavo (turkey) (BioIVT), Turdus migratorius (American robins) or Dumetella carolinensis (Gray catbirds) in the presence or absence of OmCI.

Techniques: Binding Assay, Labeling, Generated

Journal: Ticks and tick-borne diseases

Article Title: A soft tick Ornithodoros moubata salivary protein OmCI is a potent inhibitor to prevent avian complement activation

doi: 10.1016/j.ttbdis.2019.101354

Figure Lengend Snippet: A low passage, infectious, and serum resistant B. burgdorferi strain B31-5A4 (“B31-5A4”) or a high passage, a non-infectious, and serum sensitive B. burgdorferi strain B313 (“B313”) was incubated for 4h with the serum from (A) Coturnix quail (“quail”), (B) chicken, (C) geese, (D) turkey, (E) American robins (“robins”), or (F) Gray catbirds (“catbirds”) at a final concentration of 40 % in the presence (“OmCI-serum”) or absence (“serum”) of 2 μM of OmCI. Note that the results from quail were derived from Figure 3. The heat-inactivated serum from the above-mentioned animals was included as a control (“heat-treated”). The number of motile spirochetes was assessed microscopically. The percentage of survival for those B. burgdorferi strains was calculated using the number of mobile spirochetes at 4 h post incubation normalized to that prior to the incubation with serum. The experiments were performed on three independent experiments; within each experiment, samples were run in triplicate, and the survive percentage for each experiment was calculated by averaging the results from triplicate experiments. The result shown here is the average ± standard deviation of the survival percentage from three independent experiments. (*), the significant difference (P < 0.05) of the percent survival of spirochetes between indicated groups was determined using the one-way ANOVA with post hoc Dunn’s test.

Article Snippet: The cell suspensions were then mixed with 40 % of the normal serum from human, Coturnix (quail), Gallus gallus (chickens) (Biowest, Riverside, MO), Anser anser (geese) (BioIVT, Hicksville, NY), Meleagris gallopavo (turkey) (BioIVT), Turdus migratorius (American robins) or Dumetella carolinensis (Gray catbirds) in the presence or absence of OmCI.

Techniques: Incubation, Concentration Assay, Derivative Assay, Control, Standard Deviation